The internet is filled with different methods, procedures, and workflows that can overwhelm you during data analysis, especially if this is your first time with RT-qPCR. This article is going to …

Jun 17, 2023 · There are two main ways to analyze qPCR data: double delta Ct analysis and the relative standard curve method (Pfaffl method). Both methods make assumptions and have …

Gene expression analysis by real-time qPCR has been a key enabler of a routine and robust approach for measuring gene expression in genes of interest, as well as monitoring …

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system. For some …

Watch this short video to see one of our gene expression qPCR analysis tools in action. Or, visit the GeneGlobe Data Analysis Center to see all the analysis modules.

Learn statistical analysis of qPCR data for accurate fold change detection. Ensure precision in your experiments and reliable outcomes.

Here we develop a mathematical model, termed the Common Base Method, for analysis of qPCR data based on threshold cycle values (Cq) and efficiencies of reactions (E).

Quantitative PCR (qPCR) is one of the foremost methods for detection and quantification of nucleic acid targets. The technique is also known as real-time qPCR and is not to be confused …

In order to compare various amplicon panels over multiple qPCR runs, instruments, reagents and conditions, a high-throughput data analysis method termed “dots in boxes” was developed.

In this document we present some of th e usual methods used in qPCR data analysis and a practical example using Integromics’ RealTime StatMiner, the unique software analysis …