A rainbow crosswalk in Orlando, Florida, that was part of the city’s Pulse Memorial was painted over by the state late Wednesday night. The memorial honored the 49 people fatally shot by a gunman at the Pulse LGBTQ nightclub in 2016, in what was the largest mass shooting in the country at the time.

Crews from the Florida Department of Transportation removed the rainbow colors that were painted by protesters on a crosswalk near the Pulse Nightclub. The rainbow stripes were added on Friday in protest against FDOT removing the former crosswalk memorial that honored the 49 lives lost during the 2016 massacre.